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The Development of a Pyruvate Biosensor Using Electrodeposited Polytyramine

✍ Scribed by Manihar Situmorang; J. Justin Gooding; D. Brynn Hibbert; Donald Barnett

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
99 KB
Volume
14
Category
Article
ISSN
1040-0397

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✦ Synopsis

A biosensor was constructed by covalently attaching pyruvate oxidase at the surface of electrodeposited polytyramine. The resulting biosensor is sensitive to pyruvate with a linear calibration plot in the concentration range of 0.1 ± 3.0 mM pyruvate, slope 0.42 mA mM À1 and the detection limit was 0.05 mM pyruvate (S/N 3). The use of polytyramine has shown to produce a stable biosensor with excellent reproducibility.

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