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The determination of pyrophosphate in commercial triphosphate

✍ Scribed by Herman J. Weiser

Book ID
112807196
Publisher
Springer-Verlag
Year
1957
Tongue
English
Weight
489 KB
Volume
34
Category
Article
ISSN
0003-021X

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✍ VΓ©ronique Trezeguet; Bernard Labouesse πŸ“‚ Article πŸ“… 1986 πŸ› Elsevier Science 🌐 English βš– 513 KB

Quantitative determination of tryptophan at the picomole level is described, using the ATP-[32P]PPi isotopic exchange reaction catalyzed by tryptophanyl-tRNA synthetase. Sensitivity limits of 500 fmol were obtained. The presence of other amino acids at a 1000-fold excess over tryptophan did not inte