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Determination of Triphosphate and Pyrophosphate by Isotope Dilution

✍ Scribed by Quimby, O. T.; Mabis, A. J.; Lampe, H. W.

Book ID
126161291
Publisher
American Chemical Society
Year
1954
Tongue
English
Weight
873 KB
Volume
26
Category
Article
ISSN
0003-2700

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Quantitative determination of tryptophan at the picomole level is described, using the ATP-[32P]PPi isotopic exchange reaction catalyzed by tryptophanyl-tRNA synthetase. Sensitivity limits of 500 fmol were obtained. The presence of other amino acids at a 1000-fold excess over tryptophan did not inte