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The determination of base ratios of very small samples of ribonucleic acid using thin layer chromatography

✍ Scribed by Rachel M. Leech; T.A. Dyer; Christine E. Poole; Wendy Smith

Publisher
Elsevier Science
Year
1968
Tongue
English
Weight
546 KB
Volume
34
Category
Article
ISSN
1873-3778

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A simple thin-layer chromatography metho
A simple thin-layer chromatography method for base ratio analysis of small samples of RNA
✍ Joao P. Monjardino πŸ“‚ Article πŸ“… 1967 πŸ› Elsevier Science 🌐 English βš– 189 KB

Base ratio analysis of small amounts of RNA isolated from human specimens requires a sensitive and reproducible method at the level of 5-10 pg of hydrolyzates. We have found that a modification of the method described by Smith and Markham (1) meets both these requirements. Complete separation in 2.