✦ LIBER ✦

A simple thin-layer chromatography method for base ratio analysis of small samples of RNA

✍ Scribed by Joao P. Monjardino

Publisher: Elsevier Science
Year: 1967
Tongue: English
Weight: 189 KB
Volume: 21
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(67)90194-7

No coin nor oath required. For personal study only.

✦ Synopsis

Base ratio analysis of small amounts of RNA isolated from human specimens requires a sensitive and reproducible method at the level of 5-10 pg of hydrolyzates. We have found that a modification of the method described by Smith and Markham (1) meets both these requirements.

Complete separation in 2.5 hr at room temperature instead of the 2 days previously required (1) has been achieved using thin layers of cellulose under appropriate conditions and microgram amounts of hydrolyzates.

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