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The detection of Θ antigen on cells by immunofluorescence: Failure to detect Θ antigen on rosette-forming cells

✍ Scribed by S. M. Russell; M. Ferrarini; A. Munro; P. J. Lachmann

Publisher
John Wiley and Sons
Year
1972
Tongue
English
Weight
439 KB
Volume
2
Category
Article
ISSN
0014-2980

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✦ Synopsis

Abstract

A technique is described which is capable of detecting Θ antigen on the surface of lymphoid cells. It utilizes the fixation of C6 deficient rabbit complement, the fixed C3 being detected by means of anti‐C3 serum conjugated with fluorochrome.

In an attempt to demonstrate antigen‐binding cells of thymic origin, the technique has been combined with the suspension‐centrifugation technique for rosette formation. This system gives no evidence for the existence of thymusderived rosette‐forming cells.

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