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The detection of virus DNA sequences in a herpes type 2 transformed hamster cell line (333-8-9)

✍ Scribed by A. C. Minson; M. E. Thouless; R. P. Eglin; G. Darby

Publisher
John Wiley and Sons
Year
1976
Tongue
French
Weight
638 KB
Volume
17
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Herpes simplex virus type 2 DNA labelled in vitro with ^125^I has been used as a probe to search for virus DNA sequences in the 333‐8‐9 line of transformed primary hamster cells and in clones derived from the line. Virus DNA sequences were present when the cells were examined initially but these sequences were lost on subsequent handling. No virus sequences were detected in clones derived from the line. It is suggested that the presence of virus DNA is an unstable characteristic of the cell line and that these sequences may be lost at cell division. The possibility that a small fragment of the genome remains cannot be excluded.

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