The detection of phosphates on chromatograms

✍ Scribed by H. Rosenberg

• Publisher: Elsevier Science
• Year: 1959
• Tongue: English
• Weight: 289 KB
• Volume: 2
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)86331-7

✦ Synopsis

A number of procedures have been described for the detection of organic phosphates on paper chromatograms. Most methods involve the use of an acid molybdate reagent, with subsequent reduction by one of a variety of reducing agents (cf. HETTLERL). The most commonly employed are those of HANES AND ISHERWOOD*, where the reducing agent is hydrogen sulfide, and of BANDURSKI AND AXELROD~, where reduction is accomplished by ultraviolet irradiation.. A procedure for the detection of phosphate spots on chromatograms without the use of molybdic acid has been described by WADE AND MORGAN*.

The present communication deals with an alternative method for the chemical reduction of, phosphomolybdate spots on paper: the method has proved satisfactory for the detection of a variety of phosphate compounds.