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Nanogram Detection of Phospholipids on Thin-Layer Chromatograms

✍ Scribed by Johannes Müthing; Mark Radloff


Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
234 KB
Volume
257
Category
Article
ISSN
0003-2697

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A sensitive immunochemical method was developed for the detection of glycosphingolipids on thin-layer chromatograms. The procedure involves oxidation of diol groups of glycosphingolipids with sodium periodate, derivatization of the formed aldehyde groups with digoxigenin-hydrazide, and reaction of t