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Differentiation of saturated and unsaturated phospholipids on thin layer chromatograms

✍ Scribed by Touchstone, J. C. ;Levin, S. S. ;Dobbins, M. F. ;Carter, P. J.


Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
155 KB
Volume
4
Category
Article
ISSN
0935-6304

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A simple method of blotting glycosphingolipids from a high-performance thin-layer chromatography (HPTLC) plate to a polyvinylidene difluoride (PVDF) membrane is described. The developed HPTLC plate is dipped in a solvent mixture (isopropanol/0.2\% \(\mathbf{C a C l}_{2}\) / methanol, \(40 / 20 / 7\)