The detection of HIV-1 proviral DNA by PCR in clotted blood specimens
β Scribed by Yu.S. Boriskin; J.C. Booth; S. Fernando; D. Carrington; M.R. Evans; P. Hay; A.R.M. Coates
- Book ID
- 113256471
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 583 KB
- Volume
- 52
- Category
- Article
- ISSN
- 0166-0934
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A simple method was developed for measuring human immunodeficiency virus type 1 (HIV-1) proviral DNA in mononuclear cells based on the commercially available Amplicor(TM) HIV-1 polymerase chain reaction (PCR) assay and the limiting dilution method. The lowest limit of detection was four proviral gen
An internally controlled high-input PCR method, termed HIV-1 Mega-PCR was developed to lower the detection limit of HIV-1 DNA polymerase chain reaction (PCR) and to improve its value as a complementary diagnostic test. It is based on PCR amplification of two target sequences in the gag gene of HIV-1