Detection of low copy numbers of HIV-1 p
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Jürg Böni; Cyril Shah; Markus Flepp; Ruedi Lüthy; Jörg Schüpbach
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Article
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2003
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John Wiley and Sons
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English
⚖ 153 KB
An internally controlled high-input PCR method, termed HIV-1 Mega-PCR was developed to lower the detection limit of HIV-1 DNA polymerase chain reaction (PCR) and to improve its value as a complementary diagnostic test. It is based on PCR amplification of two target sequences in the gag gene of HIV-1