The Design of Biodegradable Microcarriers for Induced Cell Aggregation

✍ Scribed by Huaping Tan; Jindan Wu; Dejuan Huang; Changyou Gao

• Book ID: 102934336
• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 612 KB
• Volume: 10
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.200900160

✦ Synopsis

Abstract

PLLA microspheres were aminolyzed in hexanediamine/propanol solution to introduce free amino groups on their surface, which were further transferred into aldehyde groups by a treatment of glutaraldehyde. Chitosan__‐graft‐lactose was then covalently coupled via Schiff base formation. Morphological variation and chitosan‐graft‐__lactose immobilization were characterized. In vitro culture of rabbit auricular chondrocytes demonstrated that the PLLA microcarriers could effectively support the cell attachment and particularly induce cell aggregation on their surface. The formed cell aggregates/microcarriers composite showed higher viability and extracellular matrix production. Thus, the PLLA microcarriers can be potentially used as an injectable delivery system for cartilage repair.

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