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The cytosol origin of macromolecules extruded by cultured chick embryo fibroblast cells

โœ Scribed by David H. Adams; Alison A.G. McIntosh


Book ID
107770468
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
746 KB
Volume
16
Category
Article
ISSN
0020-711X

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## Abstract A new spectrofluorometric method for heme quantitation in cultured fibroblasts is described. The method includes: (1) heme extraction by methanol/sulfuric acid, (2) partial purification of heme by a microchromatographic method, and (3) treatment of the purified heme by oxalic acid follo