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Plaque formation by avian infectious bronchitis virus in primary chick embryo fibroblast cells in the presence of trypsin

โœ Scribed by K. Otsuki; M. Tsubokura


Publisher
Springer Vienna
Year
1981
Tongue
English
Weight
358 KB
Volume
70
Category
Article
ISSN
1432-8798

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## Abstract A new spectrofluorometric method for heme quantitation in cultured fibroblasts is described. The method includes: (1) heme extraction by methanol/sulfuric acid, (2) partial purification of heme by a microchromatographic method, and (3) treatment of the purified heme by oxalic acid follo