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The cytoplasmic tail of invariant chain modulates antigen processing and presentation

✍ Scribed by Tone F. Gregers; Tommy W. Nordeng; Hanne C. G. Birkeland; Inger Sandlie; Oddmund Bakke

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
287 KB
Volume
33
Category
Article
ISSN
0014-2980

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✦ Synopsis

Abstract

The MHC class II‐associated invariant chain (Ii) has several important functions in antigen presentation. In this study, we have examined the effect of Iip33 expression on endocytic transport and antigen presentation. We find that degradation of both endocytosed antigen and Ii itself is delayed in cells expressing high levels of Ii, whereas a mutant Ii with an altered charge distributionin the cytoplasmic tail was unable to exert this effect. Furthermore, the Ii mutant did not enhance the presentation of an Ii‐dependent MHC class II‐restricted epitope to the same extent as the wild type. In a parallel study, we investigated the effect of charge in the cytoplasmic tail of Ii. We find that due to exposed negative charges, it promotes endosome fusion events, and we suggest thatthis causes endosomal retention (Nordeng et al., Mol. Biol. Cell 2002). Together, the data reveal an additional property of the Iip33 cytoplasmic tail that contributes to the modulation of antigen processing and presentation.

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Proteolysis of the invariant chain (li) leads to the generation of abundant MHC class II-associated invariant chain peptides (CLIP), which bind in the MHC class II binding groove via supermotifs in a manner similar to that of antigenic peptides. We have engineered an li vector with the capacity to e