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The cytoplasmic appearance of three functions expressed during the G0→G1→S transition is nucleus-dependent

✍ Scribed by Gerald J. Jonak; Renato Baserga

Book ID: 102885417
Publisher: John Wiley and Sons
Year: 1980
Tongue: English
Weight: 662 KB
Volume: 105
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041050217

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✦ Synopsis

tsAF8, ts13, tsHJ-4, and TK-tsl3 cells are G,-specific temperature-sensitive (ts) mutants of BHK cells that do not enter S phase when serumstimulated from quiescence at nonpermissive temperature (39.6 '-40.6 "). TK-tsl3 are, in addition, defective in thymidine kinase. Different G, functions must be involved in these cells, since the first three cell lines complement each other when forming heterokaryons.

We have used these cells to study the role of the nucleus in the cytoplasmic expression of these G, functions during the transition of cells from the nonproliferating to the proliferating state. We fused cytoplasts from either serumstarved (Go) or serum-stimulated ( S ) tsAF8 cells with Go-ts13, Go-tsHJ-4, and Go-TK-ts13 recipient cells and determined, after serum stimulation of the fusion products, which type of cytoplasts could complement the defective G, functions. Cytoplasts from S-tsAF8 cells complemented all three functions, i.e., cybridoids between S phase cytoplasts and ts13 or tsHJ-4 recipient cells entered S at the

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