Cyclic 3',5'-nucleotide phosphodiesterase, which was originally purified from beef heart (1)) has since been reported to occur in the soluble fraction from a variety of tissues (2-5). In view of the widespread interest in the role of these phosphodiesterases in cyclic nucleotide metabolism, and the
The cyclic nucleotide phosphodiesterases of spinach chloroplasts and microsomes
โ Scribed by Eric G. Brown; Malcolm J. Edwards; Russell P. Newton; Christopher J. Smith
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 832 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0031-9422
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๐ SIMILAR VOLUMES
A procedure for the assay of cyclic nucleotide phosphodiesterase is described in which labeled cyclic nucleotide is separated from labeled nucleoside by the batchwise addition of ethanolic slurries of Dowex 2 fluoride. Under the conditions described there is no detectable adsorption of nucleoside by
## Abstract During the perinatal period, lungs undergo changes to adapt to air breathing. The genes involved in these changes are developmentally regulated by various signaling pathways, including the cyclic nucleotide cAMP. As PDE4s are critical enzymes for regulation of cAMP levels, the objective