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The Culture of Mammalian Cells on Nanostructured Silicon

✍ Scribed by Sue C. Bayliss; Robert Heald; D. Ian Fletcher; Lorraine D. Buckberry

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 270 KB
Volume: 11
Category: Article
ISSN: 0935-9648
DOI: 10.1002/(sici)1521-4095(199903)11:4<318::aid-adma318>3.0.co;2-z

No coin nor oath required. For personal study only.

✦ Synopsis

RSiCl 3 : Freshly prepared wafers were placed into a solution of the appropriate trichlorosilane (5 mM) in freshly distilled toluene for 2 h. Upon removal from the toluene solution, the wafers were rinsed with chloroform and absolute ethanol. The wafers were then baked on a hot plate at 120 C for 2 min.

Monolayer Characterization: Contact angles were determined on a Krüss Model G10 goniometer at room temperature and ambient relative humidity using the sessile drop method [21]. Monolayer thickness was determined using a Gaertner L116A ellipsometer equipped with a 632.8 nm He-Ne laser. Monolayer thicknesses were determined assuming n f = 1.5, and with the incident and reflected light making a 70 C angle to the surface normal. Data presented are averages of at least ten different measurements on three different areas of the wafer. Film roughness was determined in contact mode AFM and calculated using PSI ProScan software.

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