Induction of micronuclei in cultured mammalian cells by fume condensates of roofing asphalt

✍ Scribed by H.–W. Qian; T. Ong; W.–Z. Whong

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 484 KB
• Volume: 29
• Category: Article
• ISSN: 0271-3586
• DOI: 10.1002/(sici)1097-0274(199605)29:5<554::aid-ajim16>3.0.co;2-#

✦ Synopsis

A cwnsiderable number oj workers in the Unired Srutes u w employed in asphalt industries and are potentidly exposed to usphalt fumes. The informatioil regurdirig the potentid cari:inog~wir. 1iuzurd.F qf such fitme.r to exposed workrrs is still limited. Studies have been conditcted to determine the cyrogenetic rffects of roofing asphalt fume using cultured mammuliuii crlls. Expotientially growing Chinese krinisrer lung fibroblasts (V79 cells) were exposed to different concentriilions of condensates of type I and type 111 roofing usphult fumes. generured ut temperatiires sitnilnr to actual roofing operation (316 k 10°C). The frequencies o j micronuclecited cells in the> treiited arid control cultures were determined. Additiotiully. immutit,fluorescent staining of kinetochore wirh humun onti-kinetocliore prim u n ontibody arid,floiiresceinated goat anti-hrimun IgC wa.s used to investigate the potential mechan ism of miimnucleus ,frwmarion. The resitlts show that both types of roofing usphalt ,firme condensutes caused a significant increase in the frequency o f tnicronucleated cells, and thrit 7V70 of micronuclruted cells induced bv asphalt fume condensates curried kinetochoreposiiive rnicrorurclei. These $tidings indictite thut both ~! p e I and type IIl roofing aspllnlt firrnes ure cupuble of causing priricipallj~ cytogeneric drtniuge by spindle upparotus alterutiotis in ciiltiirrd mammalian cells. @ I Y V ~ WiIur-Liu. Inc.