The crystallization of lysozyme in the system of ionic liquid [BMIm][BF4]-water

✍ Scribed by Xinxin Li; Xiaodong Xu; Yuanyuan Dan; Jing Feng; Lan Ge; Milin Zhang

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 254 KB
• Volume: 43
• Category: Article
• ISSN: 0232-1300
• DOI: 10.1002/crat.200800040

✦ Synopsis

Abstract

Lysozyme crystallization was conducted in the ionic liquid (IL) 1‐butyl‐3‐methylimidizolium tetrafluoroborate ([BMIm][BF~4~]) with different buffer/IL proportions. It was found that the addition of [BMIm][BF~4~] could promote the crystallization process, during which more lager single crystals with controllable morphologies could be obtained due to the manageable crystal growth velocity. A probable explanation was proposed based on the influence of the ionic polarization and kinetics in the lysozyme crystallization. Moreover, the transform in coordination number and the relative growth rate of different crystal faces were discussed. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)