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The crystal structure of Escherichia coli purine nucleoside phosphorylase: a comparison with the human enzyme reveals a conserved topology

✍ Scribed by Chen Mao; William J Cook; Min Zhou; George W Koszalka; Thomas A Krenitsky; Steven E Ealick


Book ID
114329532
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
674 KB
Volume
5
Category
Article
ISSN
0969-2126

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Structure of a mutant human purine nucle
✍ Sepideh Afshar; Michael R. Sawaya; Sherie L. Morrison πŸ“‚ Article πŸ“… 2009 πŸ› Cold Spring Harbor Laboratory Press 🌐 English βš– 622 KB

## Abstract A double mutant of human purine nucleoside phosphorylase (hDM) with the amino acid mutations Glu201Gln:Asn243Asp cleaves adenosine‐based prodrugs to their corresponding cytotoxic drugs. When fused to an anti‐tumor targeting component, hDM is targeted to tumor cells, where it effectively