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The conversion of mevalonic acid into gibberellin A12-aldehyde in a cell-free system fromCucurbita pepo

โœ Scribed by J. E. Graebe; D. H. Bowen; J. MacMillan

Publisher
Springer-Verlag
Year
1972
Tongue
English
Weight
595 KB
Volume
102
Category
Article
ISSN
0032-0935

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โœฆ Synopsis

A cell-free system prepared from immature seed of Cucurbita pepo incorporates the label from mevalonate-2-1*C into ent. kaur-16.en-19-oic acid (I), , and ent-gibberell.16-en-7-al-19-oic acid (III) (gibberellin A12-aldehyde ). The products were identified by gas liquid chromatography and by combined gas chromatography-mass spectrometry of the methylated and trimethylsilylated fractions. The radioactivity of the compounds was established by reerystallisation to constant specific radioactivity. Gibberellin A12 (IV), also detected in the system after incubation by combined gas chromatographymass spectrometry may be an artefact, derived from gibberellin A12-aldehyde by a non-enzymatic conversion.

With gibberellin A,2-aldehyde, the cell-free biosynthesis of an ent-gibberellane has been achieved for the first time.

๐Ÿ“œ SIMILAR VOLUMES

The biosynthesis of a C19-gibberellin fr
The biosynthesis of a C19-gibberellin from mevalonic acid in a cell-free system from a higher plant
โœ J. E. Graebe; P. Hedden; P. Gaskin; J. MacMillan ๐Ÿ“‚ Article ๐Ÿ“… 1974 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 135 KB

Incubation of a cell-free system from immature seeds of Cucurbita maxima Duch. with [(14)C]GA12-aldehyde derived from [(14)C]mevalonic acid in the same system yielded the C19-gibberellin, [(14)C]GA4, in addition to the C20-gibberellins, [(14)C]GA37, [(14)C]GA13 and [(14)C]GA43. (GA-gibberellin).