The fate of the carbon-20 atom in gibberellin (GA) biosynthesis was studied in a cell-free system from Pisum sativum. This carbon atom is lost at the aldehyde stage of oxidation when C2o-GAs are converted to C19-GAs. Gibberellin A~2 labeled with 14C at C-20 was prepared from [3"-~4C]mevalonic acid w
The biosynthesis of a C19-gibberellin from mevalonic acid in a cell-free system from a higher plant
β Scribed by J. E. Graebe; P. Hedden; P. Gaskin; J. MacMillan
- Publisher
- Springer-Verlag
- Year
- 1974
- Tongue
- English
- Weight
- 135 KB
- Volume
- 120
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
β¦ Synopsis
Incubation of a cell-free system from immature seeds of Cucurbita maxima Duch. with [(14)C]GA12-aldehyde derived from [(14)C]mevalonic acid in the same system yielded the C19-gibberellin, [(14)C]GA4, in addition to the C20-gibberellins, [(14)C]GA37, [(14)C]GA13 and [(14)C]GA43. (GA-gibberellin).
π SIMILAR VOLUMES
A cell-free system prepared from immature seed of Cucurbita pepo incorporates the label from mevalonate-2-1\*C into ent. kaur-16.en-19-oic acid (I), , and ent-gibberell.16-en-7-al-19-oic acid (III) (gibberellin A12-aldehyde ). The products were identified by gas liquid chromatography and by combined
Gibberellin (GA) metabolism from GA12-aldehyde was studied in cell-free systems from 2-d-old germinating embryos of barley. [(14)C]- or [17-(2)H2]Gibberellins were used as substrates and all products were identified by combined gas chromatography-mass spectrometry. Stepwise analysis demonstrated the
It has recently been demonstrated that the conversion of squalene into lanosterol in animal systems proceeds in two distinct steps, involving initisl oxidation of the squalene to 2,3-oxidosqualene (I), which subsequently cyclizes to