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The content of coenzyme A, acetyl-CoA and long-chain acyl-CoA in human blood platelets

โœ Scribed by Ole C. Ingebretsen; Anne M. Bakken; Mikael Farstad


Book ID
115824030
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
439 KB
Volume
126
Category
Article
ISSN
0009-8981

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A method for the extraction of acyl-CoA esters from tissue, and their subsequent analysis by HPLC is described. The lipids are removed by a two-phase extraction in a chloroform/methanol/water system. The long-chain acyl-CoA esters are extracted using methanol and a high salt concentration (2 M ammon