Determination of the intracellular conce
โ
S.Randolph May; Robert S. Krooth
๐
Article
๐
1976
๐
Elsevier Science
๐
English
โ 833 KB
The properties of an assay for the 5-phosphoribosyl-1-pyrophosphate (PRPP) content of cultured mammalian fibroblasts are described. The assay is based upon the PRPP-dependent release of "CO, from [carboxy/-"Clorotic acid by a commercially available preparation of yeast orotidine-5'-monophosphate pyr