𝔖 Bobbio Scriptorium
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The chromatographic separation of uroporphyrin I and III octamethyl esters

✍ Scribed by Jerry C. Bommer; Bruce F. Burnham; Robert E. Carlson; David Dolphin

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
341 KB
Volume
95
Category
Article
ISSN
0003-2697

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✦ Synopsis

Uroporphyrins I and III have been separated by high performance liquid chromatography on a microporasil column using n-heptaneglacial acetic acid:acetone:water (90:60:30:0.05 by volume). By using uro [3H]porphyrin I and uro [l*C]porphyrin III it was shown that less than 2% cross contamination occurred during the chromatographic separation. The same system may be used to separate uro through protoporphyrins (as their esters) by simply increasing the acetone concentration.

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