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Chromatographic Separation and Spectroscopic Characterization of the Bilirubin Isomers IIIα, IXα, XIIIα and Their Dimethyl Esters

✍ Scribed by Defoin-Straatmann, Rosalie ;Defoin, Albert ;Kuhn, Hans Joachim ;Schaffner, Kurt

Publisher: John Wiley and Sons
Year: 1982
Tongue: English
Weight: 321 KB
Volume: 1982
Category: Article
ISSN: 0947-3440
DOI: 10.1002/jlac.198219821002

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✦ Synopsis

Abstract

The three structural isomers bilirubin IXα (1a), IIIα (2a), and XIIIα (3a) have been separated on a preparative scale by high‐performance low‐pressure liquid chromatography. They were converted into their dimethyl esters 1b, 2b, and 3b and characterized by UV, IR, MS, and fully assigned ^1^H NMR data. NMR is the only spectroscopic method which makes it possible to analyze mixtures of the three isomeric dimethyl esters quantitatively.

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Complete Assignments of the 13C NMR Spec

Complete Assignments of the 13C NMR Spectra of Bilirubin-IXα and its Dimethyl Ester

✍ Norbert Müller 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 322 KB

Using two-dimensional heteronuclear 'H-13C chemical shift correlation spectroscopy via one-bond and long-range couplings, all 13C resonances of bilirubinl X a and its dimethyl ester in dimethyl sulphoxide solution are assigned, independently from reference to partial structures. Previous ambiguities