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The Choice of Reference Cell in the Analysis of Kinetic Data Using BIAcore

✍ Scribed by Raimund J. Ober; E.Sally Ward

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
152 KB
Volume
271
Category
Article
ISSN
0003-2697

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✦ Synopsis

An important step in the analysis of sensorgram data for BIAcore experiments is the subtraction of reference cell data to remove the effects of the bulk shift on the sensorgram of interest. It is shown that this step can introduce errors in the measured kinetic constants. This phenomenon is investigated both theoretically and with experimental data.

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