We analyzed blood samples from more than 200 normal adults, and quantified their Hb F by cation-exchange high-performance liquid chromatography. In several subjects with slightly elevated Hb F (0.4-4.3%), we determined the Ggamma levels in the Hb F and DNA sequence variations in the locus control re
The chemical heterogeneity of the fetal hemoglobin in normal newborn infants and in adults
β Scribed by T. H. J. Huisman; H. Harris; M. Gravely; W. A. Schroeder; J. R. Shelton; J. B. Shelton; L. Evans
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 992 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0300-8177
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## Abstract BFUβE from the blood of 14 normal adults have been grown by the plasma clot technique. The hemoglobins synthesized in burst colonies were purified from other proteins by affinity chromatography on Sepharoseβhaptoglobin. The radioactivity incorporated in the globin chains was estimated b
A variant of fetal hemoglobin (Hb F-Malta-I) has been detected and quantitated in adult blood with a sensitive radioimmunoassay employing monospecific antisera. The concentration of Hb-F-Malta-I was 0.002-0.05%, with an average value of 0.011%. The ratio of Hb F-Malta-I/Hb F in adults was about 4.8%