The characterisation of thiobarbituric acid reactivity in human plasma and urine

✍ Scribed by J.M.C. Gutteridge; T.R. Tickner

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 492 KB
• Volume: 91
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90838-2

✦ Synopsis

The specificity of the thiobarbituric acid reaction (TBA) has been investigated using techniques of high-performance thin-layer chromatography and spectrofluorimetry. It was found that malondialdehyde (MDA) derived from different lipid and nonlipid origins formed the same MDA-TBA complex. This complex could be separated from other TBA-reactive compounds by both chromatography and spectrofluorimetry. Normal human plasma and urine both formed an MDA-TBA complex along with other TBA-reactive compounds. In plasma this was associated mainly with phosphatidylcholine and appeared to be peroxidic in reaction. Urine, however, contained polar MDA-forming compounds probably resulting from the oxidation of 2-deoxyaldoses during the acid-heating stage of the TBA test.