The C.B6-H-2dm5mutant

✍ Scribed by Roger W. Melvold; Mauro S. Sandrin; Ian F. C. McKenzie

• Publisher: Springer-Verlag
• Year: 1982
• Tongue: English
• Weight: 96 KB
• Volume: 15
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00364342

✦ Synopsis

The C.B6-H-2 dins mutant strain was derived from a spontaneous gain+loss mutation recovered in a (BALB/cKh x C57BL/6Kh)F 1 female (CB6F1). It was backcrossed repeatedly to the BALB/cKh strain for ten generations before being made homozygous and has been maintained by brother x sister matings since. The screening and complementation tests by skin grafting have been previously described (Bailey and Kohn 1965, Kohn 1973, Melvold and Kohn 1976).

The mutant was complemented by B6.C-H-2 d, but not by C.B6-H-2 b indicating involvement of H-2. Complementation mapping with recombinants and mutants of the H-2 complex is shown in Table 1 where the mutation is placed in K or I-A, but most probably K since H-2 din5 fails to complement the H-2 era4 mutant, which has also been mapped to K e (Morgan et al. 1981). Skin grafts exchanged between C.B6-H-2 am5 and the parental BALB/cKh are rejected as shown in Table 2. Furthermore,