The BRCA2 transactivation domain does not interact with JNK

✍ Scribed by Gerhard H.W. May; Fiona Harris; David Gillespie; Donald M. Black

• Book ID: 101263507
• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 120 KB
• Volume: 25
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/(sici)1098-2264(199908)25:4<407::aid-gcc16>3.0.co;2-i

✦ Synopsis

The N-terminal region of BRCA2 has the capacity to activate transcription when fused to a heterologous DNA binding domain and includes a segment with amino acid similarity to the JNK-docking site in the cellular JUN protein. However, unlike JUN, we have determined that this region of BRCA2 neither interacts with nor serves as a substrate for JNK, or any other kinase that can be detected in extracts from either fibroblasts or epithelial cells. While this clearly does not rule out a transcriptional role for BRCA2, our findings indicate that BRCA2 is not regulated by the JNK pathway in a manner analogous to JUN.