## Abstract Conditions were established for routine cinemicrographic examination of sperm incorporation by living zonaβfree mouse eggs employing oil immersion objectives and Nomarski optics. Initial sperm attachment to the egg plasma membrane, which was reversible and appeared to require flagellar
β¦ LIBER β¦
The block to sperm penetration in zona-free mouse eggs
β Scribed by Don P. Wolf
- Book ID
- 118873124
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 900 KB
- Volume
- 64
- Category
- Article
- ISSN
- 0012-1606
No coin nor oath required. For personal study only.
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## Abstract The presence of trypsin inhibitors during insemination reduced penetration of zonaβintact and zonaβfree mouse ova by capacitated sperm. This inhibition was dependent upon both concentration of inhibitor and sperm. Preincubation of gametes in inhibitors did not markedly influence their s