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The biosynthesis of sinefungin: Investigations using a cell-free system

✍ Scribed by Ronald J. Parry; Shyhchen Ju

Publisher
Elsevier Science
Year
1991
Tongue
French
Weight
727 KB
Volume
47
Category
Article
ISSN
0040-4020

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✦ Synopsis

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The origin of the adenylyl moiety of sinefungin has been investigated by administration of doublylabeled forms of ATP and adenosineto cell-free extracts of Streptomyces griseohs. The resuks demonstrated that both ATP and adenosine are significantly degraded by the extract before incorpomtion into sineftmgin. However, the ribose ring of adenosine was shown to be incorporated into sinefungin intact, and without loss of tritium from C-S. This observation rules out the intemdiacy of A914SC in sinefungin biosynthesis. Additional experiments are described whiih su gest that C-C bond formation between C-S of arginine and C-5 of a ribose derivative may precede attachment of t!e adenine ring.

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