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Biosynthesis of the Indole Alkaloids. Cell-free Systems from Catharanthus roseus Plants

✍ Scribed by James P. Kutney; Lewis S. L. Choi; Toshio Honda; Norman G. Lewis; Toshitsugu Sato; Kenneth L. Stuart; Brian R. Worth

Publisher
John Wiley and Sons
Year
1982
Tongue
German
Weight
806 KB
Volume
65
Category
Article
ISSN
0018-019X

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✦ Synopsis

Abstract

Cell‐free systems from Catharanthus roseus plants are utilized for various studies relating to the biosynthesis of indole alkaloids. Tryptamine (5) and secologanin (6), two fundamental building units, are shown to be incorporated into the alkaloid vindoline (7). In another study, catharanthine (18) and vindoline (7) are utilized by this enzyme system and coupled to the important bisindole biointermediate 3′,4′‐anhydrovinblastineThe previously [20] used name for 17, 3′, 4′‐dehydrovinblastine, is incorrect.

(17). The latter substance is, in turn, incorporated and converted to the natural alkaloids leurosine (8), Catharine (9) and vinblastine (10), thereby providing information about the biosynthesis of these complex molecules. High pressure liquid chromatography assay of the enzymic mixture sheds light on the enzymes involved in the coupling of 18 and 7.

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## Abstract A cell‐free extract, which was isolated from the leaves of mature __Catharanthus roseus__ plants by a previously published procedure, __does not__ convert a mixture of secologanin and radiolabelled tryptamine to vindoline, as was recently claimed. The radioactivity in the purified alkal