This paper examines the reversed-phase liquid chromatographic behavior of ribonuclease A (RNase) using an n-butyl chemically bonded phase and a gradient of 10 mM H3PO4 and l-propanol. At a column temperature of 25 degrees C, a broad band followed by an overlapped late-eluting sharp peak is observed.
The behaviour of hydrophylic substances in reversed-phase chromatography a contribution to the mechanism of reversed-phase paper chromatography
✍ Scribed by J. Gasparič
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 773 KB
- Volume
- 47
- Category
- Article
- ISSN
- 1873-3778
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