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The basolateral membrane of rat enterocyte: Its purification from brush border contamination

✍ Scribed by M.N. Orsenigo; M. Tosco; G. Esposito; A. Faelli

Publisher: Elsevier Science
Year: 1985
Tongue: English
Weight: 521 KB
Volume: 144
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(85)90156-3

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✦ Synopsis

Basolaterai membranes obtained by self-orienting Percoll-gradient centrifugation were treated with 5 mM CaClz to minimize the cross-contamination by brush border membranes. From marker enzyme-q&tic activities it was calculated that in this preparation the basolateral/brush border membrane ratio was 22.6. A low ~-glucose permeability across basolateral membrane vesicles together with ATPdependent sodium uptake was observed. o 1985 Academic PKSS, IIIC.

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