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Partial purification of the Na+-dependentd-glucose transport system from renal brush border membranes

โœ Scribed by Wha Bin Im; Kit Yin Ling; Robert G. Faust

Publisher
Springer
Year
1982
Tongue
English
Weight
812 KB
Volume
65
Category
Article
ISSN
0022-2631

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High-affinity phlorizin binding to brush
High-affinity phlorizin binding to brush border membranes from small intestine: Identity with (a part of) the glucose transport system, dependence on the Na+-gradient, partial purification
โœ Tannenbaum, Carl ;Toggenburger, Gerhard ;Kessler, Markus ;Rothstein, Aser ;Semen ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 794 KB

In the presence of an NaSCN gradient phlorizin binds with a high affinity (Kd 4.7 pM) t o vesicles derived from brush border membranes of intestinal cells of rabbits. The value for Kd corresponds closely t o that of Ki determined from phlorizin inhibition of sugar transport. The apparent affinity fo