Reconstitution of a partially purified Na+ -independent d-glucose transport system from rat jejunal basolateral membranes

High-affinity phlorizin binding to brush

High-affinity phlorizin binding to brush border membranes from small intestine: Identity with (a part of) the glucose transport system, dependence on the Na+-gradient, partial purification

✍ Tannenbaum, Carl ;Toggenburger, Gerhard ;Kessler, Markus ;Rothstein, Aser ;Semen 📂 Article 📅 1977 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 794 KB

In the presence of an NaSCN gradient phlorizin binds with a high affinity (Kd 4.7 pM) t o vesicles derived from brush border membranes of intestinal cells of rabbits. The value for Kd corresponds closely t o that of Ki determined from phlorizin inhibition of sugar transport. The apparent affinity fo