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The automation of immun-FIA-systems

✍ Scribed by B. Hitzmann; A. Löhn; M. Reinecke; B. Schulze; T. Scheper

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
638 KB
Volume
313
Category
Article
ISSN
0003-2670

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✦ Synopsis

In this contribution an automation system for the control, measurement uptake, evaluation and visualization is presented as well as its application for two different flow-injection immunoassays.

Both systems are based on the principles of flow-injection analysis and were developed for a reliable, fast monitoring of relevant proteins in animal cell cultivation processes. One system uses a homogeneous assay with turbidimetric detection, the other a heterogeneous assay with an immobilized immunocomponent applying fluorescence detection. The on-line application during a mammalian cell cultivation as well as off-line measurements of medium samples are presented. The results obtained from on-line measurements are compared to ELISA results. The automation of the analysis systems with respect to its flexible control as well as the reliable evaluation of the measurement signals -even if they are severely distorted -are discussed in detail.

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