The assay of pterostilbene in spiked matrices by liquid chromatography tandem mass spectrometry and isotope dilution method

✍ Scribed by Fabio Mazzotti; Leonardo Di Donna; Hicham Benabdelkamel; Bartolo Gabriele; Anna Napoli; Giovanni Sindona

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 234 KB
• Volume: 45
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.1718

✦ Synopsis

Abstract

Pterostilbene (trans‐3,5‐dimethoxy‐4‐hydroxystilbene) is an active component found in several plant species, exhibiting important pharmacological properties. A new and reliable method of assaying this phyto compound in various matrices is presented; the assay is based on (1) the selectivity of liquid chromatography (LC) hyphenated with electrospray ionisation (ESI), (2) the specificity of a two‐step mass spectrometric analysis (MS/MS) and (3) the accuracy of the isotope dilution method. The labelled analogue may be conveniently synthesised in a few steps. The sensitivity of the method is confirmed by the very low limit of detection (LOD) and limit of quantitation (LOQ) values achieved in the assay of pterostilbene in two distinct fortified matrices, and is further supported by the observed accuracy values. Copyright © 2010 John Wiley & Sons, Ltd.