The art and design of genetic screens: mammalian culture cells
โ Scribed by Grimm, Stefan
- Book ID
- 109951833
- Publisher
- Nature Publishing Group
- Year
- 2004
- Tongue
- English
- Weight
- 351 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1471-0056
- DOI
- 10.1038/nrg1291
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
RSiCl 3 : Freshly prepared wafers were placed into a solution of the appropriate trichlorosilane (5 mM) in freshly distilled toluene for 2 h. Upon removal from the toluene solution, the wafers were rinsed with chloroform and absolute ethanol. The wafers were then baked on a hot plate at 120 C for 2
Ultraviolet (UV)-light and 5-fluorodeoxyuridine (FUdR), two known D N A damaging agents, were found to enhance the frequency of stable plasmid transformations in several different animal cell lines. Combined treatment with the two agents was more effective than treatment with either agent alone. A c