The Arabidopsis thaliana cDNA sequencing projects
✍ Scribed by Michel Delseny; Richard Cooke; Monique Raynal; Françoise Grellet
- Book ID
- 117107329
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 272 KB
- Volume
- 405
- Category
- Article
- ISSN
- 0014-5793
No coin nor oath required. For personal study only.
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As part of the goal to generate a detailed transcript map for __Arabidopsis thaliana__, 1152 single run sequences (expressed sequence tags or ESTs) have been determined from cDNA clones taken at random in libraries prepared from different sources of plant material: developing siliques, etiolated see
Using a kinetic approach, a cDNA library composed of almost equal representations of all genes expressed in the aerial parts of 2‐week‐old __Arabidopsis__ was constructed. A cDNA was synthesized with an oligo dT primer containing a __Not__l site. A linker containing the nucleotide sequence of __Sse_