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Efficient construction of cDNA microarrays utilizing normalized cDNA libraries of Arabidopsis thaliana

โœ Scribed by Kohei Ando; Seiyei Yamakawa; Kyoko Miyashita; Kazuya Yoshida; Akiho Yokota; Atsuhiko Shinmyo; Takayuki Kohchi

Book ID
114399673
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
197 KB
Volume
97
Category
Article
ISSN
1389-1723

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Construction of an equalized cDNA librar
Construction of an equalized cDNA library from Arabidopsis thaliana
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Using a kinetic approach, a cDNA library composed of almost equal representations of all genes expressed in the aerial parts of 2โ€weekโ€old __Arabidopsis__ was constructed. A cDNA was synthesized with an oligo dT primer containing a __Not__l site. A linker containing the nucleotide sequence of __Sse_