The application of preparative batch HPLC, supercritical fluid chromatography, steady-state recycling, and simulated moving bed for the resolution of a racemic pharmaceutical intermediate

✍ Scribed by Tony Q. Yan; Carlos Orihuela; David Swanson

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 328 KB
• Volume: 20
• Category: Article
• ISSN: 0899-0042
• DOI: 10.1002/chir.20512

✦ Synopsis

Abstract

This article discusses the chromatographic resolution of a racemic pharmaceutical intermediate. Preparative batch high performance liquid chromatography (HPLC), supercritical fluid chromatography (SFC), steady‐state recycling (SSR), and simulated moving bed (SMB) were used to resolve a total of 12.2 kg of a racemic pharmaceutical intermediate. In this study, a first batch of 0.8 kg of racemate was separated on the preparative batch HPLC and SFC, and subsequently another 5.9 kg of racemate was separated on the SSR. Lastly, a third batch of 5.5 kg was separated on the SMB. The separation conditions and results of these techniques are discussed. The productivities and solvent costs of SFC versus HPLC are compared. The productivities and solvent costs of SMB, SSR, and HPLC are also compared. The analytical method development and process optimization of these processes are also discussed in this article. Chirality, 2008. © 2007 Wiley‐Liss, Inc.