𝔖 Bobbio Scriptorium
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The application of papain, ficin and clostripain in kinetically controlled peptide synthesis in frozen aqueous solutions

✍ Scribed by Dr Marion Hänsler; Grit Ullmann; Hans-Dieter Jakubke

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
398 KB
Volume
1
Category
Article
ISSN
1075-2617

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✦ Synopsis

Abstract

The capability of the cysteine proteases ficin, papain and clostripain to form peptide bonds in frozen aqueous solutions was investigated. Freezing the reaction mixture resulted in increased peptide yields in kinetically controlled coupling of Bz–Arg–OEt with various amino acid amides and dipeptides. Under these conditions, peptide yields increased up to 70% depending on the enzyme and the amino component used. Enzyme‐catalysed peptide syntheses were carried out under optimized reaction conditions (temperature, amino component concentration and pH before freezing) using the condensation of Bz–Arg–OEt and H–Leu–NH~2~ as a model reaction.

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