An apparatus has been developed that allows photoaffinity ligands to be crossed-linked to milligram quantities of membrane proteins with maximum attainable yield following contact times of approximately 1 ms. The apparatus consisted of three parts: a conventional rapid mixing unit, a novel freeze-qu
The application of ligand overlay of protein blots to the study of the nicotinic acetylcholine receptor
โ Scribed by Dr. Jonathan M. Gershoni
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 454 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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results in an increased OD (Table 2). However, the presence of the resistant GGT allele can be directly determined using cPASA as previously described by Zhu and Clark. 7
## Abstract For Abstract see ChemInform Abstract in Full Text.
The interaction of diisopropylfluorophosphate (DFP) with the nicotinic acetylcholine (ACh) receptor of Torpedo electric organ was studied, using [3H]-phencyclidine ([3H]-PCP) as a reporter probe. Phencyclidine binds with different kinetics to resting, activated, and desensitized receptor conformatio
A consensus prediction for the secondary structure of the muscle nicotinic acetylcholine receptor (โฃ, โค, โฅ, and โฆ subunits) extracellular regions is presented. This protein is a member of the ligand-gated ion channel superfamily, which also encompasses the 5HT 3 , GABA A , and glycine receptors. The