DNA sequencing technology was modified into a quantitative assay, which for multiple DNA sequences allowed the simultaneous determination of relative protein-DNA binding constants. The band mobility shift of the protein-DNA binding reactions partitions the mixture of DNA sequences into bound and unb
The APC protein binds to A/T rich DNA sequences
✍ Scribed by Deka, Jürgen; Herter, Peter; Sprenger-Haußels, Markus; Koosch, Sonja; Franz, Dorothee; Müller, Klaus-Michael; Kuhnen, Cornelius; Hoffmann, Ingrid; Müller, Oliver
- Book ID
- 110062342
- Publisher
- Nature Publishing Group
- Year
- 1999
- Tongue
- English
- Weight
- 913 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0950-9232
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## Summary The external spacer (ES) of rRNA nuclear genes (rDNA) contains the sequences that control rDNA transcription initiation and enhancement. The ES is also characterized in most species by the presence of multiple repeated elements. In higher plants very few data are available on the __cis‐_
We investigated DNA-protein-interactions occurring in the promoter region of c-fos using twodimensional electrophoresis and south-western-blotting. When nuclear extracts from the human glioblastoma cell line HeRoSV were tested for their DNA-binding behaviour to a 650 bp-fragment within the promoter