𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A set of 4 nuclear proteins binds to a DNA sequence within the FOS promoter region

✍ Scribed by Janna Radtke; Steven Dooley; Nikolaus Blin; Gerhard Unteregger

Publisher
Springer
Year
1991
Tongue
English
Weight
873 KB
Volume
15
Category
Article
ISSN
0301-4851

No coin nor oath required. For personal study only.

✦ Synopsis

We investigated DNA-protein-interactions occurring in the promoter region of c-fos using twodimensional electrophoresis and south-western-blotting. When nuclear extracts from the human glioblastoma cell line HeRoSV were tested for their DNA-binding behaviour to a 650 bp-fragment within the promoter region of c-fos, we found 4 proteins designated as 120/6.6, 75/5.4, 65/6.4 55/5.0 interacting with this fragment. An additional protein 60/6.0 was detected by using a digoxygenine-labelled probe. These observations let us to assume that beside the well characterized SRF and FOS-JUN proteins additional factors recognize the promoter sequence and may play a role in c-fos regulation.

πŸ“œ SIMILAR VOLUMES

A nuclear protein binding to dA/dT-rich
A nuclear protein binding to dA/dT-rich sequences upstream from the radish DMA promoter
✍ Manuel Echeverria; Dominique Delcasso-Tremousaygue; Michel Delseny πŸ“‚ Article πŸ“… 1992 πŸ› John Wiley and Sons 🌐 English βš– 937 KB

## Summary The external spacer (ES) of rRNA nuclear genes (rDNA) contains the sequences that control rDNA transcription initiation and enhancement. The ES is also characterized in most species by the presence of multiple repeated elements. In higher plants very few data are available on the __cis‐_

Characterization of adjacent E-box and n
Characterization of adjacent E-box and nuclear factor 1-like DNA binding sequence in the human CYP1A2 promoter
✍ Marta J. Narvaez; Garret R. Anderson; George V. Pickwell; Linda C. Quattrochi πŸ“‚ Article πŸ“… 2005 πŸ› John Wiley and Sons 🌐 English βš– 196 KB πŸ‘ 1 views

## Abstract To better understand the molecular mechanisms of cytochrome P450 1A2 (__CYP1A2__) regulation, we have characterized a region of the promoter (+3 to βˆ’176) that contains a single E‐box and an adjacent nuclear factor 1 (NF1)‐like DNA binding site. The E‐box was shown to specifically bind n

A 110-kD nuclear shuttling protein, nucl
A 110-kD nuclear shuttling protein, nucleolin, binds to the neurite-promoting IKVAV site of laminin-1
✍ M. C. Kibbey; B. Johnson; R. Petryshyn; M. Jucker; H. K. Kleinman πŸ“‚ Article πŸ“… 1995 πŸ› John Wiley and Sons 🌐 English βš– 940 KB

## Abstract The basement membrane protein laminin and the IKVAV‐containing sequence from the laminin Ξ±l chain have been found to promote the differentiation of primary neurons and a variety of neural cell lines. We previously reported that a 110‐kd IKVAV‐binding protein (LBP110) isolated from brain

Identification of transcription factor i
Identification of transcription factor in the promoter region of rat regucalcin gene: Binding of nuclear factor I-A1 to TTGGC motif
✍ Hiroyuki Misawa; Masayoshi Yamaguchi πŸ“‚ Article πŸ“… 2002 πŸ› John Wiley and Sons 🌐 English βš– 222 KB πŸ‘ 1 views

## Abstract Hepatic nuclear protein has been reported to bind specifically to the TTGGC sequence of the rat regucalcin gene promoter region in stimulating the promoter activity (Misawa and Yamaguchi [2000] Biochem. Biophys. Res. Commun. 279: 275–281). The present study was undertaken to identify tr